Soluble DHFR expression concentrations in molecules for every mobile measured from lysate activity assays as described in Materials and solutions.
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assortment coefficients for –Lon range (Figure 1—resource details one) as compared to monoculture advancement costs calculated within a plate reader in ER2566 ∆folA/∆thyA (–Lon) as explained in products and strategies.
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The situation from the DHFR gene is detailed in parenthesis in the primary column. Expression values corresponds to your cell pressure inside the column heading.
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(2020) Altered expression of an outstanding Management protease in E. coli reshapes the in vivo mutational landscape of a design enzyme